budding stage meaning in English
脱苞期
Examples
- Thl1 gene was also amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 )
采用pcr和rt - pcr技术，从甘蓝和油菜基因组dna和柱头总rna中扩增获得了719bp的thl1基因和430bp的cdna序列。 - Thl2 gene was finally amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) . the dna sequencing result shows that the genomic gene of thl2 is approximately 900 bp from brassica oleracea l . in contrast to that of 850 bp from brassica napus l .
采用阿r和rtpcr技术，从甘蓝和油菜基因组和柱头总则a中扩增获得了thlz基因，基因组中得到的基因大约为900hp和850hp ， cdna大约为477hp 。在thlz基因中首次发现了两个内含于，并且甘蓝和油菜thlz基因的内含于序列同源性低于ich ，并且大小也有差异。 - The method of ctab was specifically improved for the extraction of genomic dna and total rnas from brassica oleracea l . and brassica napus l . 2 . arclboler gene was amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 )
以甘蓝( 200110197 )和油菜( y578 - 2 )自交不亲和系的植株为材料，提取幼苗中的gdna及花蕾期的柱头、子房、花瓣、叶片、根的mrna ，并对mrna进行反转录合成cdna ，然后分别以gdna与cdna为模板扩增arc1蛋白编码序列。